Signalling

Part:BBa_K5316015:Design

Designed by: Santiago Ramos Bartolome   Group: iGEM24_Barcelona-UB   (2024-09-30)


Lck signal peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 86
    Illegal PstI site found at 12
    Illegal PstI site found at 91
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 86
    Illegal PstI site found at 12
    Illegal PstI site found at 91
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 86
    Illegal BamHI site found at 117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 86
    Illegal PstI site found at 12
    Illegal PstI site found at 91
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 86
    Illegal PstI site found at 12
    Illegal PstI site found at 91
    Illegal AgeI site found at 123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ATGGGCTGTGGCTGCAGCTCAAACCCTGAAGATGACTGGATGGAGAACATTGACGTGTGTGAGAACTGCCATTATCCCGGCGGACGAATTCTGCAGTCGACGGTACCGCGGGCCCGGGATCCACCGGTG


Source

Sequence sourced from J Neurosci Methods. 2005 Jan 30;141(1):41-53: https://pubmed.ncbi.nlm.nih.gov/15585287/

References